October 11, 2019 4 p.m.End:
October 11, 2019 4 p.m.Location: Cooper Nursing Building, Room 160
Department of Biology
Biology Fall Seminar Series: Visualizing Neuronal Circuits in Fruit Flies
Friday, Oct. 11 | 4-5 p.m. | Cooper Nursing 160 | Free & open to campus community
Presenter: Dr. Douglas Roossien, Ball State University, Department of Biology
Topic: A novel toolkit for studying serotonergic neuron development in Drosophila
Summary: Proper wiring of neuronal circuits during development is crucial for forming a functional nervous system, and aberrations in the cellular processes that form these circuits underlies many behavioral and cognitive disorders. While the genetic tractability and simplicity of the fruit fly (Drosophila) has propelled its use as a model organism, traditional approaches for analyzing structural components of the nervous system have limited our ability to determine outcomes from genetic manipulations on the cellular and circuit levels. We have recently developed new tools that allow entire neuronal circuits to be visualized and quantified. Briefly, the combination of multispectral labeling (Brainbow), expansion microscopy, and neuron tracing software allows us to assess how genetic manipulation of cellular processes impacts neuronal circuit development in whole, intact Drosophila nervous systems. We are currently using this pipeline to determine the role of serotonin autoregulation on axon growth and branching in serotonergic neurons.