Biology 457 & 557 - Molecular Biology
Tentative Lab Topic Schedule
There will be instances when lecture time is spent in lab
and vice versa. Such instances will be announced ahead of time in class.
You will also be required to perform lab duties outside of class time, as
molecular techniques are time-intensive!
Bolded lab exercises denote the ones to be prepared as a lab report (3 total).
FALL SEMESTER 2008
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| Week 1 (Aug. 27):
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Introduction/Prepare Stock
Solutions/Instrument Usage |
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| Week 2 (Sept. 3): |
Sterile Cell Culture
Techniques/Growth of Adherent Cells/Practice Cell
Counts |
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| Week 3 (Sept. 10): |
Continuation of Cell Culturing – Cell Counts/Freezing Cells |
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| Week 4 (Sept. 17): |
Bacterial Transformation
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| Week 5 (Sept. 24): |
Midi-Preps of Plasmid
DNA |
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| Week 6 (Oct. 1): |
Transfection of
Cells |
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| Week 7 (Oct. 8): |
RNAi/siRNA |
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| Week 8 (Oct. 15): |
Indirect Apoptotic Assay
(TUNEL Method) |
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| Week 9 (Oct. 22): |
View
Apoptotic Slides |
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| Week 10 (Oct. 29): |
Extraction of
Nuclear and Cytoplasmic Protein |
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| Week 11 (Nov. 5): |
Protein
Determination/Standard Curves |
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| Week 12 (Nov. 12): |
Western
Analysis |
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| Week 13 (Nov. 19): |
Western Analysis (cont.) |
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| Week 14 (Nov. 26): |
NO LAB - Thanksgiving Break |
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| Week 15 (Dec. 3): |
Caspase
Activity Assay |
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| Week 16 (Dec. 10): |
Wrap Up Lab/Student
Presentations |