Biology 113  Dr. J. McKillip 
Fall 2004  Laboratory Exercises
 

Date Day Topic
     
1-11 T Introduction to lab; scientific techniques & serial dilution intro
     
1-13 TH Scientific techniques - standard curve
     
1-18 T Biomolecules
     
1-20 TH Factors influencing enzyme activity
     
1-25 T Cellular reproduction: mitosis & meiosis
     
1-27 TH DNA isolation fro a salt-loving microorganism
     
2-01 T Introduction to the microscope; wet mounts of protozoa; prepared slides
     
2-03 TH Microscopic observation of yeast & molds
     
2-08 T Universal nature of microorganisms; streak plate; SPC on contaminated milk
     
2-10 TH Introduction to the microscope: smear prep & prepared slides
     
2-15 T Gram stain
     
2-17 TH Spore stain
     
2-22 T Acid-Fast stain; streak plate revisited
     
2-24 TH Sel./diff. media: MSA, EMB, MacConkey, PR glu/lac/suc/mal/man broths, TSI
     
3-01 T Sel./diff. media: citrate, SIM, nitrate, urea, catalase, orn. decarb., SSA, MR-VP, thioglycolate, start agar
     
3-04 TH Sel/diff.media cont'd ;  orn. decarb.; SSA; MR-VP; thioglycolate; starch agar
     
3-8-3-10 T-TH No Class - Spring Break
     
3-15 T Staph/strep/coagulase/BAP, MSA, SM110
     
3-17 TH Kirby-Bauer assay & antibiotic susceptibility
     
3-22 T UV light effects on bacterial growth
     
3-24 TH Ames Test
     
3-29 T Begin Unknown ID
     
3-31 TH UV light effects on bacterial growth
     
11-02 T Ames test
     
11-04 TH  
     
11-09 T E-Test on throat/nasal isolates
     
11-11 TH PPT on current methods in moleculardiagnostics
     
11-16 T Water analysis:  MPN determination
     
11-18 TH SPC revisited: contaminated drinking water
     
11-23 T Eukaryotic microbiology I:  fungi/slide cultures
     
11-25 TH No Class - Thanksgiving
     
11-30 T Eukaryotic microbiology II: yeast
     
12-02 TH Eukaryotic microbiology III:  protozoa
     
12-07 T Food microbiology:  making yogurt
     
12-09 TH Lab Practical