Biot 491 591

This course will provide both theory and laboratory experiences using the Polymerase Chain Reaction (PCR) technique. Students will amplify DNA and RNA from tissues of various organisms to explore the potential applications of this versatile technique. Topics include computer design of primers, heat stable DNA Polymerases, optimization of conditions, amplification oAn introduction to research design & presentation, providing discussions on how to define an original research problem, access needed information, employ rigorous experimental design, use appropriate statistical analyses, follow scientific assumptions/approaches, and submit the proposal in written and oral forms. Working independently, students will design a research proposal and include expected results. Individuals will submit a written proposal and make a PowerPoint presentation to the class.f sequences from cloned genes, mRNA, and genomic sequences, subcloning of PCR products, and use of PCR in differential display, and quantitation of mRNA levels.

Course Title:	BIOT 491/591 - Theory & Applications of the Polymerase Chain Reaction
Semester Offered:	Fall
Days / Time:	MF / 11:00 - 13:00
Credits:	3
Instructor:	Dr. Claire Chatot (cchatot@bsu.edu) or Dr.John McKillip jlmckillip@bsu.edu
Prerequisites:	Cell Biology (Intro to Recombinant DNA recommended)
Synopsis:	This course will provide both theory and laboratory experiences using the Polymerase Chain Reaction (PCR) technique. Students will amplify DNA and RNA from tissues of various organisms to explore the potential applications of this versatile technique. Topics include computer design of primers, heat stable DNA Polymerases, optimization of conditions, amplification oAn introduction to research design & presentation, providing discussions on how to define an original research problem, access needed information, employ rigorous experimental design, use appropriate statistical analyses, follow scientific assumptions/approaches, and submit the proposal in written and oral forms. Working independently, students will design a research proposal and include expected results. Individuals will submit a written proposal and make a PowerPoint presentation to the class.f sequences from cloned genes, mRNA, and genomic sequences, subcloning of PCR products, and use of PCR in differential display, and quantitation of mRNA levels.